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Results of Search
| BCRJ: | 0093 | | Cell Line: | GR | | Tissue: | CONNECTIVE | | Organ: | LIVER | | Cell Type: | FIBROBLAST | | Morphology: | FIBROBLAST | | Pathology: | SCHISTOMIASIS | | Scientific Name: | MUS MUSCULUS | | Vulgar Name: | MOUSE; C3H/HE | | Sex: | | | Miscelaneous Info: | | | Virus Succeptibility: | | | Virus Resistance: | | | Tumor Formation: | | | Products: | Dermatan sulfate; heparan sulfate. | | Dependency Isoenzymes: | | | Culture Medium: | Dulbecco's MEM 90%, fetal bovine serum 10%.
Subculture procedure: standard trypsinization techniques.
A split ratio of 1:2 or 1:4 is recommended. | | Consigner: | Helio dos Santos Druta, Departamento de Bioquimica, Instituto de
Quimica, UFRJ, Rio de Janeiro. | | Reference: | J. Exp. Pathology, 71: 845-856, 1990. | | Additional Info: | GR cell line was obtained from spontaneous migration of cells from
granulomas induced in livers of C3H/Hej mice infected with
Schistosoma mansoni. These cells display myofibroblast
characteristics, and represent murine liver connective tissue cells.
They present instability in chromosome number during subsequent
passages. They are thought to give rise to continous cell line, GRX
(BCRJ-CR001), after a number of passages with significant change in
morphology and proliferation rate. | | ATCC: | | | Biosafety: | 01 |
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