| BCRJ: | 0090 |
| Cell Line: | GH3 |
| Tissue: | EPITHELIUM |
| Organ: | PITUITARY GLAND |
| Cell Type: | EPITHELIAL |
| Morphology: | EPITHELIAL |
| Pathology: | TUMOR |
| Scientific Name: | RATTUS NORVEGICUS |
| Vulgar Name: | RAT; WISTAR/FURTH RAT |
| Sex: | FEMALE |
| Miscelaneous Info: | 7 MONTH OLD |
| Virus Succeptibility: | HERPES SIMPLEX; VESICULAR STOMATITIS (INDIANA STRA |
| Virus Resistance: | POLIOVIRUS 1 |
| Tumor Formation: | |
| Products: | Prolactin; growth hormone |
| Dependency Isoenzymes: | |
| Culture Medium: | Ham's F10 medium 82.5%, horse serum 15%, fetal bovine serum 2.5%.
Subculture procedure: standard trypsinization techniques.
A split ratio 1:2 to 1:4 is recommended |
| Consigner: | Dr. Guilherme Kurtz, Laboratorio de Farmacologia Celular, UFRJ,
Rio de Janeiro. |
| Reference: | J. Cell Biol. 43: 432-, 1969;
Exp. Cell Res. 64: 125-128, 1971. |
| Additional Info: | GH3 cells was established from a pituitary tumor of a 7 month old
female Wistar/Furth rat. GH3 cells produce growth hormone at agreater rate than
GH1 cells and also produces prolactin. Studies on GH3 have
shown that hydrocortisone stimulates hormone production and
inhibits prolactin. The cells are capable of adaption to growth in
suspension culture using MEM. |
| ATCC: | CCL 82.1 |
| Biosafety: | 01 |
