| BCRJ: | 009 |
| Cell Line: | 11 B 11; BSF-1 |
| Tissue: | NEURAL |
| Organ: | |
| Cell Type: | LYMPHOBLAST |
| Morphology: | LYMPHOBLAST |
| Pathology: | HYBRIDOMA |
| Scientific Name: | RATTUS NORVEGICUS |
| Vulgar Name: | RAT; LEW/SN |
| Sex: | |
| Miscelaneous Info: | |
| Virus Succeptibility: | Cells contain SV-40 Viral DNA sequences |
| Virus Resistance: | |
| Tumor Formation: | |
| Products: | Immunoglobulin; monoclonal antibody; BSF-1: ab against; IL-4: ab against;
Interleukin-4: ab agaisnt; IgG1. |
| Dependency Isoenzymes: | |
| Culture Medium: | RPMI-1640 with L-glutamine 90%, fetal bovine serum 10%.
Subculture procedure: maintain cultures from 5x104 to 5x105 cells/mL. |
| Consigner: | Dr. Ises A. Abrahamsohn, Departamento de Imunologia, ICB, USP,
São Paulo |
| Reference: | Nature 315: 333-336, 1985. |
| Additional Info: | The line was produced by fusing P3-NS-1/1-Ag4-1 myeloma cells
with spleen cells from Lew/Sn rats imunized with purified BSF-1
emulsified in complete Freund's adjuvant. The 11B11 cell line
secretes a rat monoclonal antibody ( IgG1 ) that reacts with murine
interleukin-4 ( IL-4 ) = B cell stimulatory factor-1 ( BSF-1 ). The antibody
does not cross-react with other lymphokines, and can be used to
purify BSF-1 from complex mixtures. May be distributed to scientific
intitutions, not to be distributed for any commercial use. |
| ATCC: | HB-188 |
| Biosafety: | 01 |
