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BCRJ:	0085
Cell Line:	F98
Tissue:	HEMATOPOIETIC
Organ:	BRAIN
Cell Type:	B LYMPHOCYTE
Morphology:	GLIAL
Pathology:
Scientific Name:	RATTUS NORVEGICUS
Vulgar Name:	RAT
Sex:
Miscelaneous Info:
Virus Succeptibility:
Virus Resistance:
Tumor Formation:	YES
Products:
Dependency Isoenzymes:
Culture Medium:	The base medium for this cell line is Dulbecco's Modified Eagle's Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended Medium Renewal: Every 2 to 3 days Storage temperature: liquid nitrogen vapor phase
Consigner:	DEBORA AMADO INFAR- BIOQUIMICA
Reference:	38592: Ko L, et al. Morphological characterization of nitrosourea-induced glioma cell lines and clones. Acta Neuropathol. 51: 23-31, 1980. PubMed: 7435138 38606: Tzeng JJ, et al. Adoptive immunotherapy of a rat glioma using lymphokine-activated killer cells and interleukin 2. Cancer Res. 50: 4338-4343, 1990. PubMed: 2364388 38610: Barth RF, et al. Boron Neutron capture therapy of brain tumors: Enhanced survival following intracarotid injection of either sodium borocaptate or boronophenylalanine with or without blood brain barrier disruption. Cancer Res. 57: 1129-1136, 1997. PubMed: 9067283 38611: Clendenon NR, et al. Boron neutron capture therapy of a rat glioma. Neurosurgery 26: 47-55, 1990. PubMed: 2294479 38612: Matalka KZ, et al. Neutron capture therapy of a rat glioma using boronophenylalanine as a capture agent. Radiat. Res. 137: 44-51, 1994. PubMed: 8265787 38613: Tzeng JJ, et al. Phenotype and functional activity of tumor-infiltrating lymphocytes isolated from immunogenic and nonimmunogenic rat brain tumors. Cancer Res. 51: 2373-2378, 1991. PubMed: 2015600 38614: Gyngell ML, et al. Localized proton NMR spectroscopy of experimental gliomas in rat brain in vivo. NMR Biomed. 5: 335-340, 1992. PubMed: 1336973 38615: Bockhorst K, et al. Localization of experimental brain tumors in MRI by gadolinium porphyrin. Acta Neurochir. Suppl. (Wien) 60: 347-349, 1994. PubMed: 7976586 38616: Els T, et al. NMR contrast enhancement of brain tumors: Comparison of the blood brain barrier tracer GdDTPA and the tumor-selective contrast agent in MnTPPS. MAGMA 1: 126-133, 1993. 38617: Hossmann KA, et al. Regional metabolism of experimental brain tumors. Acta Neuropathol. 69: 139-147, 1986. PubMed: 3962590 38621: Eis M, et al. High resolution quantitative relaxation and diffusion MRI of three different experimental brain tumors in rat. Magn. Reson. Med. 34: 835-844, 1995. PubMed: 8598810 38622: Eis M, et al. Quantitative diffusion MR imaging of cerebral tumor and edema. Acta Neurochir. Suppl. (Wien) 60: 344-346, 1994. PubMed: 7976585 38623: Judy KD, et al. Effectiveness of controlled release of a cyclophosphamide derivative with polymers against rat gliomas. J. Neurosurg. 82: 481-486, 1995. PubMed: 7861228 38624: Ernestus RI, et al. Identification of intracranial liqor metastases of experimental stereotactically implanted brain tumors by the tumor-selective MRI contrast agent MnTPPS. Clin. Exp. Metastasis 10: 345-350, 1992. PubMed: 1505124 38625: Yang W, et al. Boron neutron capture therapy of brain tumors: enhanced survival following intracarotid injection of sodium borocaptate with or without blood-brain barrier disruption. Int. J. Radiat. Oncol. Biol. Phys. 37: 663-672, 1997. PubMed: 9112465 38626: Yang W, et al. Enhanced survival of glioma bearing rats following boron neutron capture therapy with blood-brain barrier disruption and intracarotid injection of boronophenylalanine. J. Neuro-Oncol. 33: 59-70, 1997. PubMed: 9151224 38627: Boucher Y, et al. Interstitial fluid pressure in intracranial tumours in patients and in rodents. Br. J. Cancer 75: 829-836, 1997. PubMed: 9062403 38629: Barba D, et al. Tumor immunity, immunizations and herpes simplex virus-thymidine kinase mediated tumor regression. Abstr. 1st Scientific Meeting Soc. Neuro-Onco. : 1996.. 39799: Vinores SA, Koestner A. The effect of nerve growth factor on undifferentiated glioma cells. Cancer Lett. 10: 309-318, 1980. PubMed: 7427924 39800: Kobayashi N, et al. An improved rat brain-tumor model. J. Neurosurg. 53: 808-815, 1980. PubMed: 7003068 39801: Barth RF. Rat brain tumor models in experimental neuro-oncology: the 9L, C6, T9, F98, RG2 (D74), RT-2 and CNS-1 gliomas. J. Neuro-Oncol. 36: 91-102, 1998. PubMed: 9525831
Additional Info:	This tumor was produced by Drs. A. Koestner and W. Wechsler at The Ohio State University in 1971. A pregnant CD Fischer rat on day 20 of gestation was inoculated with a single dose of N-ethyl-N-nitrosourea (ENU) (50 mg/kg b.w.). Tumors were harvested and cloned. [38592] This cell line may be used for both in vitro and in vivo studies of a rat brain tumor. It grows well in cell culture and provides a simple, reproducible glioma model when inoculated into the brains of syngeneic rats. The biological characteristics of this tumor closely resemble those of human glioblastoma. The transplantable tumor displays an infiltrative pattern of growth within the brain. [38611] It is weakly immunogenic in syngeneic rats and an intracerebral inoculum of as few as 100 cells are lethal. [38613] The F98 and RG2 (ATCC CRL-2433) gliomas can be used as rat brain tumor models in experimental neuro-oncology. [39801] F98 was submitted to the American Type Culture Collection in June, 1998 at passage 2 from a culture established from a subcutaneous tumor grown in a male Fischer rat.
ATCC:	CRL-2397
Biosafety:	1