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Results of Search
| BCRJ: | 0079 | | Cell Line: | EOMA | | Tissue: | | | Organ: | | | Cell Type: | ENDOTHELIAL | | Morphology: | ENDOTHELIAL | | Pathology: | HEMANGIOENDOTHELIOMA | | Scientific Name: | MUS MUSCULUS | | Vulgar Name: | MOUSE | | Sex: | | | Miscelaneous Info: | | | Virus Succeptibility: | | | Virus Resistance: | | | Tumor Formation: | YES | | Products: | angiotensin converting enzyme (ACE) [51514]
thrombospondin [51514]
cathepsin L [47148]
endostatin [47148]
interleukin-6 (interleukin 6, IL-6) [53484] | | Dependency Isoenzymes: | | | Culture Medium: | The base medium for this cell line is Dulbecco's Modified Eagle's Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Storage temperature: liquid nitrogen vapor phase | | Consigner: | TATIANA SAMPAIO
UFRJ | | Reference: | 47148: Felbor U, et al. Secreted cathepsin L generates endostatin from collagen XVIII. EMBO J. 19: 1187-1194, 2000. PubMed: 10716919
51514: Obeso J, et al. A hemangioendothelioma-derived cell line: its use as a model for the study of endothelial cell biology. Lab. Invest. 63: 259-269, 1990. PubMed: 2166185
53484: Zhu XL, et al. Kinetics of cytokine gene expression in macrophage and endothelial cell lines following liposome encapsulated haemoglobin (LEH) treatment in vitro. Cytokine 8: 541-547, 1996. PubMed: 8891435
53486: Wen W, et al. The generation of endostatin is mediated by elastase.. Cancer Res. 59: 6052-6056, 1999. PubMed: 10626789 | | Additional Info: | The EOMA cell line was originally derived in 1980 from a mixed hemangioendothelioma arising in an adult mouse. [51514]
The cells synthesize angiotensin-converting enzyme, express surface receptors for acetylated low density lipoprotein, produce thrombospondin and show intracellular staining with an antibody to von Willebrand factor. [51514]
Cathepsin L is secreted by EOMA cells and is responsible for the generation of endostatin L. [47148]
Although constitutive cytokine gene expression exists in EOMA cells, the level of IL-6 mRNA is prominently elevated by incubation with Liposome encapsulated hemoglobin (LEH). [53484]
The cells constitutively express the vascular addressin identified by antibody MECA-99.
EOMA cells exhibit characteristic endothelial cell properties, such as rearrangement into tubelike structures on Matrigel and retention of cobblestone morphology at confluence. They behave in vitro in a manner similar to microvascular endothelial cells. [51514] | | ATCC: | CRL-2586 | | Biosafety: | 1 |
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