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Results of Search
| BCRJ: | 0077 | | Cell Line: | DH 82 | | Tissue: | HEMATOPOIETIC | | Organ: | | | Cell Type: | MONOCYTE-MACROPHAGE | | Morphology: | MACROPHAGE-LIKE | | Pathology: | | | Scientific Name: | CANIS FAMILIARIS | | Vulgar Name: | CANINE ( GOLDEN RETRIEVER ) | | Sex: | | | Miscelaneous Info: | The DH 82 cell line has a macrophage like morphology, and is able to phagocytize latex particles. They are positive for Fc gamma receptors and negative for Fc mu and C3b receptors. | | Virus Succeptibility: | | | Virus Resistance: | | | Tumor Formation: | | | Products: | | | Dependency Isoenzymes: | | | Culture Medium: | MEM (Eagle) with 2mM L-glutamine and Earle’s BSS, 0,1 mM nonessential amino acids, and 1.0 mM sodium pyruvate, 85%; heat inactivated fetal bovine serum, 15 %. A subcultivation ratio of 1:4 to 1;8 is recommended. | | Consigner: | Cláudio Luiz Massard, Departamento de Parasitologia Animal Universidade Federal Rural do Rio de Janeiro | | Reference: | Dawsonn JE and Rikihisa Y. Growing Ehrlichia species ina continuous cell line. U.S.Pat. 5,192,679 dated Mar. 9,1993 | | Additional Info: | Growth Properties: adherent | | ATCC: | CRL 10389 | | Biosafety: | 01 |
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