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BCRJ:	0074
Cell Line:	DA-1
Tissue:	HEMATOPOIETIC
Organ:	BONE MARROW
Cell Type:	MYELOID
Morphology:	LYMPHOBLAST
Pathology:
Scientific Name:	MUS MUSCULUS
Vulgar Name:	MOUSE
Sex:
Miscelaneous Info:
Virus Succeptibility:
Virus Resistance:
Tumor Formation:
Products:
Dependency Isoenzymes:	IL-3; interleukin-3; or GM-CSF; GM-colony stimulating factor
Culture Medium:	RPMI-1640 90%, fetal bovine serum 10%, 2mM L-glutamine, 2mM sodium pyruvate, and a source of IL-3 and/or GM-CSF. Maintain cultures from 5x10 exp4 to 5x10 exp5 cells/mL (further information on request).
Consigner:	Dr. Marcelo Barciski, Instituto de Biofisica, CCS, UFRJ, Rio de Janeiro.
Reference:	Dr. Marcelo Barciski, Instituto de Biofisica, CCS, UFRJ, Rio de Janeiro.
Additional Info:	The DA-1 cell line is dependent upon IL-3 (interleukin-3). It can be used to quantify the presence of IL-3 in biological fluids. Grows in suspension.
ATCC:
Biosafety:	01