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BCRJ:	0072
Cell Line:	CRFK
Tissue:	EPITHELIUM
Organ:	KIDNEY
Cell Type:	EPITHELIAL
Morphology:	EPITHELIAL-LIKE
Pathology:	NORMAL
Scientific Name:	FELIS CATTUS
Vulgar Name:	CAT
Sex:
Miscelaneous Info:	The cells are free of bovine viral diarrhea (BVD) virus
Virus Succeptibility:	feline reovirus; canine parvovirus; rabiesvirus; bovine viral diarrhea (BVD) virus
Virus Resistance:
Tumor Formation:
Products:
Dependency Isoenzymes:
Culture Medium:	Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; horse serum, 10%. Subculture procedure: standard trypsinization techniques. A split ratio 1:3 to 1:8 is recommended
Consigner:	Maurício José Fernades, Vencofarma do Brasil
Reference:	Crandell RA , et al. Development, characterization, and viral susceptibility of a feline (Felis catus) renal cell line (CRFK). In Vitro 9: 176-185, 1973. Fabricant CG , et al. Microbial studies of feline urolithiasis. J. Am. Vet. Med. Assoc. 158: 976-980, 1971. Lee KM , et al. Use of an established cat cell line for investigation and quantitation of feline oncornaviruses. J. Natl. Cancer Inst. 49: 55-60, 1972. Loffler S , et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997.
Additional Info:	Virus Succeptibility feline herpesvirus; feline calicivirus; feline panleukopenia. Growth Properties: adherent
ATCC:	CCL 94
Biosafety:	01