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| BCRJ: | 0071 | | Cell Line: | COS-7 | | Tissue: | | | Organ: | KIDNEY | | Cell Type: | HUMAN ACUTE PROMYELOCYTIC LEUKEMIA | | Morphology: | TRANSFECT FIBROBLAST | | Pathology: | TRANSFECT | | Scientific Name: | CERCOPITHECUS AETHIOPS | | Vulgar Name: | MONKEY | | Sex: | | | Miscelaneous Info: | ADULT | | Virus Succeptibility: | | | Virus Resistance: | | | Tumor Formation: | | | Products: | T-antigen | | Dependency Isoenzymes: | | | Culture Medium: | Dulbecco's MEM 90%, fetal bovine serum 10%.
Subculture procedure: standard trypsinization techniques.
A split ratio of 1::4t o 1:8 is recommended.
Medium Renewal: 2 to 3 times per week | | Consigner: | Antonio Martins Monteiro - Banco de Células do Rio de Janeiro | | Reference: | 1822: Gluzman Y. SV40-transformed simian cells support the replication of early SV40 mutants. Cell 23: 175-182, 1981. PubMed: 6260373
32447: Fernandez LM, Puett D. Lys583 in the third extracellular loop of the lutropin/choriogonadotropin receptor is critical for signaling. J. Biol. Chem. 271: 925-930, 1996. PubMed: 8557706
32459: Maestrini E, et al. A family of transmembrane proteins with homology to the MET-hepatocyte growth factor receptor. Proc. Natl. Acad. Sci. USA 93: 674-678, 1996. PubMed: 8570614
32500: Campbell M, et al. The simian foamy virus type 1 transcriptional transactivator (Tas) binds and activates an enhancer element in the gag gene. J. Virol. 70: 6847-6855, 1996. PubMed: 8794326
32502: Gonzalez Armas JC, et al. DNA immunization confers protection against murine cytomegalovirus infection. J. Virol. 70: 7921-7928, 1996. PubMed: 8892915
32547: Jang SI, et al. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J. Biol. Chem. 271: 24105-24114, 1996. PubMed: 8798649
32566: Dittrich E, et al. A di-leucine motif and an upstream serine in the interleukin-6 (IL-6) signal transducer gp130 mediate ligand-induced endocytosis and down-regulation of the IL-6 receptor. J. Biol. Chem. 271: 5487-5494, 1996. PubMed: 8621406
32568: Lee JH, et al. The proximal promoter of the human transglutaminase 3 gene. J. Biol. Chem. 271: 4561-4568, 1996. PubMed: 8626812
32720: Chen Y, et al. Demonstration of binding of dengue virus envelope protein to target cells. J. Virol. 70: 8765-8772, 1996. PubMed: 8971005 | | Additional Info: | This is an African green monkey kidney fibroblast-like cell line suitable for transfection by vectors requiring expression of SV40 T antigen. This line contains T antigen, retains complete permissiveness for lytic growth of SV40, supports the replication of ts A209 virus at 40C, and supports the replication of pure populations of SV40 mutants with deletions in the early region.The line was derived from the CV-1 cell line (ATCC ® CCL-70?) by transformation with an origin defective mutant of SV40 which codes for wild type T antigen. | | ATCC: | CRL-1651 | | Biosafety: | 2 |
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