| BCRJ: | 0069 |
| Cell Line: | CHO-K1 |
| Tissue: | EPITHELIUM |
| Organ: | OVARY |
| Cell Type: | EPITHELIAL |
| Morphology: | EPITHELIAL |
| Pathology: | |
| Scientific Name: | CRICETULUS GRISEUS |
| Vulgar Name: | HAMSTER, CHINESE |
| Sex: | |
| Miscelaneous Info: | The CHO-K1 cell line was derived as a subclone from the parental CHO cell line initiated from a biopsy of an ovary of an adult Chinese hamster by T. T. Puck in 1957.
The cells require proline in the medium for growth. |
| Virus Succeptibility: | vesicular stomatitis (Indiana); Getah virus. |
| Virus Resistance: | Poliovirus 2; modoc virus; Button Willow virus. |
| Tumor Formation: | |
| Products: | |
| Dependency Isoenzymes: | |
| Culture Medium: | Ham's F12K medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%.Subculture procedure: standard trypsinization techniques. A split ratio 1:4 to 1:8 is Recommended |
| Consigner: | Leda dos Reis Castilho, Laboratório de Cultura de Células COPPE Universidade Federal do Rio de Janeiro |
| Reference: | Kao FT , et al. Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells. Proc. Natl. Acad. Sci. USA 60:
25977: Kao FT , et al. Genetics of somatic mammalian cells. IV. Properties of Chinese hamster cell mutants with respect to the requirement for proline. Genetics 55: Kao FT , et al. Complementation analysis on virus-fused Chinese hamster cells with nutritional markers. Science 164: |
| Additional Info: | Growth Properties: adherent |
| ATCC: | CCL-61 |
| Biosafety: | 01 |
