| BCRJ: | 0067 |
| Cell Line: | CHO 1-15 |
| Tissue: | |
| Organ: | OVARY |
| Cell Type: | B LYMPHOCYTE |
| Morphology: | EPITHELIAL |
| Pathology: | NORMAL |
| Scientific Name: | CRICETULUS GRISEUS |
| Vulgar Name: | CHINESE HAMSTER |
| Sex: | FEMALE |
| Miscelaneous Info: | |
| Virus Succeptibility: | |
| Virus Resistance: | |
| Tumor Formation: | |
| Products: | human tissue plasminogen activator (tPA, t-PA) |
| Dependency Isoenzymes: | |
| Culture Medium: | Complete growth medium: Ham's F12 medium suplemented with 10 % fetal bovine serum.
Subcultivation Ratio: 1:5 to 1:10 is recommended
Medium Renewal: Add fresh medium at the time of subculture
Storage temperature: liquid nitrogen vapor phase |
| Consigner: | Antonio Martins Monteiro - Banco de Células do Rio de Janeiro |
| Reference: | 2404: Goeddel DV, et al. Human tissue plasminogen activator. US Patent 4,766,075 dated Aug 23 1988 |
| Additional Info: | The line was produced by transfecting CHO cells with a plasmid (pETPFR, ATCC 40403) to produce a system in which human tissue plasminogen activator is produced.
Expresses human tissue plasminogen activator (t-PA) in eukaryotic DHFR deficient cell lines grown in selective media (glycine, hypoxanthine, thymidine deficient). [2404]
Contains a EcoRI/BamHI fragment of about 2.2 kb containing t-PA and a 1.7 kb SacII fragment containing wild-type dihydrofolate reductase under SV40 control. [2404] |
| ATCC: | CRL-9606 |
| Biosafety: | 1 |
