| BCRJ: | 0065 |
| Cell Line: | CF |
| Tissue: | CONNECTIVE |
| Organ: | HEART |
| Cell Type: | FIBROBLAST |
| Morphology: | FIBROBLAST |
| Pathology: | NORMAL |
| Scientific Name: | RATTUS NORVEGICUS |
| Vulgar Name: | RAT |
| Sex: | |
| Miscelaneous Info: | ADULT RAT |
| Virus Succeptibility: | |
| Virus Resistance: | |
| Tumor Formation: | |
| Products: | |
| Dependency Isoenzymes: | |
| Culture Medium: | Dulbecco¦s Modified Eagle Medium (DMEM) suplemented with 10% (v/v) fetal calf
serum. Standard tripsinization techniques. Split ratio 1:2 to 1:3 recommended. |
| Consigner: | Flavio Henrique P. Braga
Banco de Celulas do Rio de Janeiro
Programa Avançado de Biologia Celular Aplicado a Medicina - PABCAM
Hospital Universitario Clementino Fraga Filho - UFRJ |
| Reference: | |
| Additional Info: | This primary cell culture were obtained by tripsin diggestion of entire rat heart
followed by collagenase type II treatment. Cells were partially purified by diferential
adesion to eliminate myocites for 2 hours and the supernadant plated after that.
Complete isolation protocol avaiable under request. |
| ATCC: | |
| Biosafety: | 01 |
