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| BCRJ: | 0061 | | Cell Line: | CCD-1059Sk | | Tissue: | | | Organ: | SKIN | | Cell Type: | FIBROBLAST | | Morphology: | FIBROBLAST | | Pathology: | NORMAL | | Scientific Name: | HOMO SAPIENS | | Vulgar Name: | HUMAN | | Sex: | FEMALE | | Miscelaneous Info: | | | Virus Succeptibility: | | | Virus Resistance: | | | Tumor Formation: | | | Products: | | | Dependency Isoenzymes: | | | Culture Medium: | The base medium for this cell line is Eagle's Minimum Essential Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 3 to 4 days
Storage temperature: liquid nitrogen vapor phase | | Consigner: | VIVIAN ZAGUE
GELITA DO BRASIL LTDA | | Reference: | | | Additional Info: | The line was established from skin taken from normal breast tissue removed at biopsy.
Cells from the current passage (1) have experienced at least 2 population doublings beyond the biopsy material, and senesce after approximately 34 additional population doublings.
DNA Profile (STR):
Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 9,11
D5S818: 11
D7S820: 11,12
THO1: 9,9.3
TPOX: 8
vWA: 16,18
Isoenzymes:
G6PD, B | | ATCC: | CRL-2072 | | Biosafety: | 1 |
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