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BCRJ:	0058
Cell Line:	C2C12
Tissue:	MUSCULAR
Organ:
Cell Type:	MYELOBLAST
Morphology:	MYOBLASTIC-LIKE
Pathology:
Scientific Name:	MUS MUSCULUS
Vulgar Name:	MOUSE
Sex:
Miscelaneous Info:
Virus Succeptibility:
Virus Resistance:
Tumor Formation:
Products:
Dependency Isoenzymes:
Culture Medium:	Dulbecco's MEM with 4.5 g/L glucose 90%, fetal bovine serum 10%. Subculture procedure: standard tripsinization techniques. A split ratio from 1:2 to 1:4 is recommended. Do not let cells reach 70% of confluency!
Consigner:	Dr. Enrique Brandan, Department of Cell and Molecular Biology, Faculty of Biological Sciences, Catholic University of Chile.
Reference:	Nature ( London ) 270: 725-727, 1977; Science ( Washington, DC ) 230: 758-766, 1985.
Additional Info:	The C2C12 is a subclone derived by H. Blau, et al. from mouse myoblast cell line established by D. Yaffe and O. Saxel from normal adult C3H mouse leg muscle. The cell line differentiates rapidly and produces extensive contracting myotubes expressing characteristic muscle proteins. The clone provides an excellent model to study myogenesis in vitro and cell differentiation. May be distributed to scientific institutions; not to distributed for any commercial use.
ATCC:	CRL-1772
Biosafety:	01