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| BCRJ: | 0057 | | Cell Line: | C 6 | | Tissue: | | | Organ: | | | Cell Type: | B LYMPHOCYTE | | Morphology: | FIBROBLAST | | Pathology: | | | Scientific Name: | RATTUS NORCEGICUS | | Vulgar Name: | | | Sex: | | | Miscelaneous Info: | | | Virus Succeptibility: | | | Virus Resistance: | | | Tumor Formation: | | | Products: | S-100 protein; produce glyceryl phosphate dehydrogenase in response to glucocorticoids; somatotrophin | | Dependency Isoenzymes: | | | Culture Medium: | Ham’s F12 medium with 2mM L-glutamine, 82,5%; horse serum, 15%; fetal bovine serum 2,5%. Subculture procedure: standard trypsinization techniques. A split ratio 1:2 to 1:3 is recommended. | | Consigner: | Ana Oliveira Battastini, ICB Universidade Federal do Rio de Janeiro | | Reference: | Benda P , et al. Differentiated rat glial cell strain in tissue culture. Science 161: 370-371, 1968. 25965: Lightbody JJ , et al. Establishment of differentiated clonal strains of glial brain cells in culture. Fed. Proc. 27: 720, 1968. 32720: Chen Y , et al. Demonstration of binding of dengue virus envelope protein to target cells. J. Virol. 70: 8765-8772, 1996. | | Additional Info: | Tissue glioma, brain; glial cell Organ brain; glia cell
The glial cell strain, C6, was cloned a rat glial tumor induced by N-nitrosomethylurea by Benda et al. after a series of alternative culture and animal passages.
Growth Properties: adherent | | ATCC: | CCL 107 | | Biosafety: | 01 |
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