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| BCRJ: | 0051 | | Cell Line: | BHK-570 | | Tissue: | | | Organ: | KIDNEY | | Cell Type: | FIBROBLAST | | Morphology: | FIBROBLAST | | Pathology: | | | Scientific Name: | MESOCRICETUS AURATUS | | Vulgar Name: | HAMSTER, SYRIAN GOLDEN | | Sex: | | | Miscelaneous Info: | The BHK-570 cell line is a baby hamster kidney cell line that is thymidine kinase deficient (TK-) [PubMed: 9153209]. The cells are useful as hosts for transfection studies. Used in producing a mammalian G protein coupled glutamate receptor [U.S. Pat. 5,385,831]. | | Virus Succeptibility: | | | Virus Resistance: | | | Tumor Formation: | | | Products: | | | Dependency Isoenzymes: | | | Culture Medium: | Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; fetal bovine serum, 10%.
Subculture procedure: standard tripsinization techniques.
A split ratio 1:4 to 1:10 is recommended. | | Consigner: | Márcio Poças - Universidade de Brasília | | Reference: | 4351: Mulvihill ER , et al. Method for producing a mammalian G protein copuled glutamate receptor. US Patent 5,385,831 dated Jan 31 1995 | | Additional Info: | | | ATCC: | CRL-10314 | | Biosafety: | 01 |
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