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| BCRJ: | 0050 | | Cell Line: | BHK-21(C-13) | | Tissue: | | | Organ: | KIDNEY | | Cell Type: | B LYMPHOCYTE | | Morphology: | FIBROBLAST | | Pathology: | NORMAL | | Scientific Name: | MESOCRICETUS AURATUS | | Vulgar Name: | HAMSTER; GOLDEN SYRIAN HAMSTER | | Sex: | | | Miscelaneous Info: | NEONATAL | | Virus Succeptibility: | ADENOVIRUS 25; HERPES SIMPLEX; REOVIRUS 3; VESICUL | | Virus Resistance: | POLIOVIRUS 2 | | Tumor Formation: | | | Products: | | | Dependency Isoenzymes: | | | Culture Medium: | Dulbecco's MEM 90%, fetal bovine serum 10%.
Subculture procedure: standard trypsinization technique.
A split ratio 1:5 is recomended.
Doubling time: ca. 32-50 hours | | Consigner: | Leda dos Reis Castilho, Federal University of Rio de Janeiro, COPPE, Laboratory Cell Culture Technology Centro de Tecnologia Bloco G sala 116 55 21 2562.8336 | | Reference: | Virology 16: 147-151, 1962. MacPherson et al., Virology 16: 147-151 (1962), PubMed ID 14468055
MacPherson, J Natl Cancer Inst 30: 795-811 (1963), | | Additional Info: | Although generally considered to be fibroblast, BHK cell line
bears a number of characteristics of the smooth muscle cell
lineage. The clone S-13 was selected for good growth in
suspension. This is not the frequently used clone BHK-21(C-13).
DSMZ no.: ACC 61
Origin: BHK-21 is a subclone (clone 13) of the parental line established from the kidneys of five unsexed, one-day-old hamsters in 1961; cells can be used for virus replication studies | | ATCC: | CCL-10 | | Biosafety: | 01 |
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