Results of Search

BCRJ:	0047
Cell Line:	BALB/3T3 clone A31
Tissue:
Organ:	EMBRYO
Cell Type:	FIBROBLAST
Morphology:	FIBROBLAST
Pathology:
Scientific Name:	MUS MUSCULUS
Vulgar Name:	MOUSE
Sex:
Miscelaneous Info:
Virus Succeptibility:	Herpes simplex virus Vesicular stomatitis virus Herpes simplex virus Vesicular stomatitis virus
Virus Resistance:
Tumor Formation:	NO
Products:
Dependency Isoenzymes:
Culture Medium:	The base medium for this cell line is Dulbecco's Modified Eagle's Medium. To make the complete growth medium, add the following components to the base medium: bovine calf serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C Growth Conditions: The serum used is calf serum, NOT fetal calf serum. The depositor recommended calf serum because fetal calf serum causes transformation and loss of contact inhibition. Storage temperature: liquid nitrogen vapor phase Subcultivation Ratio: For 60 mm plates, use an inoculum of 3 X 10(5) cells per plate and subculture every 3 days. Never allow cultures to become completely confluent before subculture. Medium Renewal: Twice per week
Consigner:	KELEN ARROTEIA NATURA
Reference:	22708: Aaronson SA, Todaro GJ. Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40. J. Cell. Physiol. 72: 141-148, 1968. PubMed: 4301006 26022: Todaro GJ, Aaronson SA. Properties of clonal lines of murine sarcoma virus transformed Balb-3T3 cells. Virology 38: 174-202, 1969. PubMed: 4306523 26023: Aaronson SA, Todaro GJ. Basis for the acquisition of malignant potential by mouse cells cultivated in vitro. Science 162: 1024-1026, 1968. PubMed: 4301647 26024: Jainchill JL, Todaro GJ. Stimulation of cell growth in vitro by serum with and without growth factor. Relation to contact inhibition and viral transformation. Exp. Cell Res. 59: 137-146, 1970. PubMed: 4194429 32554: Thompson SA, et al. COOH-terminal extended recombinant amphiregulin with bioactivity comparable with naturally derived growth factor. J. Biol. Chem. 271: 17927-17931, 1996. PubMed: 8663535 32907: Anderson MT, et al. Simultaneous fluorescence-activated cell sorter analysis of two distinct transcriptional elements within a single cell using engineered green fluorescent proteins. Proc. Natl. Acad. Sci. USA 93: 8508-8511, 1996. PubMed: 8710900 92346: Biological evaluation of medical devices. Part 5: Tests for in vitro cytotoxicity. Sydney, NSW, Australia:Standards Australia;Standards Australia AS ISO 10993.5-2002. 92389: Biological evaluation of medical devices--Part 5: Tests for in vitro cytotoxicity. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 10993-5:1999.
Additional Info:
ATCC:	CCL-163
Biosafety:	1