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BCRJ: | 0046 | Cell Line: | B16-F10 | Tissue: | | Organ: | SKIN | Cell Type: | MELANOCYTE | Morphology: | FIBROBLAST | Pathology: | MELANOMA | Scientific Name: | MUS MUSCULUS | Vulgar Name: | MOUSE; C57BL/6J | Sex: | | Miscelaneous Info: | | Virus Succeptibility: | | Virus Resistance: | | Tumor Formation: | IN C57BL/6J MOUSE | Products: | | Dependency Isoenzymes: | | Culture Medium: | Dulbecco's MEM 90%, fetal bovine serum 10%. Standard
trypsinization techniques. Cultures may be passaged by scraping
the cells from the flask, dispersing them by rapid pipetting and
seeding into new flasks with fresh medium. | Consigner: | Instituto Nacional do Cancer, Rio de Janeiro. | Reference: | Nature New Biol. 242: 148-149, 1973;
Cancer Res. 35: 218, 1975;
Cancer Res. 36: 3608, 1976;
Cancer Res. 37: 3945, 1977;
Science 197: 893, 1977;
J. Nat. Cancer Inst. 60: 12117-22, 1978. | Additional Info: | The B16-F10 melanoma cell line was extensively used for
studies of cancer metastasis. The B16-F1 line is a standard
clone usually compared to B16-F10 clone that was selected for
its high capacity to form lung metastasis. | ATCC: | CRL-6475 | Biosafety: | 01 |
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