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BCRJ: 0046
Cell Line: B16-F10
Tissue:
Organ: SKIN
Cell Type: MELANOCYTE
Morphology: FIBROBLAST
Pathology: MELANOMA
Scientific Name: MUS MUSCULUS
Vulgar Name: MOUSE; C57BL/6J
Sex:
Miscelaneous Info:
Virus Succeptibility:
Virus Resistance:
Tumor Formation: IN C57BL/6J MOUSE
Products:
Dependency Isoenzymes:
Culture Medium: Dulbecco's MEM 90%, fetal bovine serum 10%. Standard trypsinization techniques. Cultures may be passaged by scraping the cells from the flask, dispersing them by rapid pipetting and seeding into new flasks with fresh medium.
Consigner: Instituto Nacional do Cancer, Rio de Janeiro.
Reference: Nature New Biol. 242: 148-149, 1973; Cancer Res. 35: 218, 1975; Cancer Res. 36: 3608, 1976; Cancer Res. 37: 3945, 1977; Science 197: 893, 1977; J. Nat. Cancer Inst. 60: 12117-22, 1978.
Additional Info: The B16-F10 melanoma cell line was extensively used for studies of cancer metastasis. The B16-F1 line is a standard clone usually compared to B16-F10 clone that was selected for its high capacity to form lung metastasis.
ATCC: CRL-6475
Biosafety: 01


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