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BCRJ: | 0045 | Cell Line: | B16-F1 | Tissue: | | Organ: | SKIN | Cell Type: | MELANOCYTE | Morphology: | FIBROBLAST | Pathology: | MELANOMA | Scientific Name: | MUS MUSCULUS | Vulgar Name: | MOUSE; C57BL/6J | Sex: | | Miscelaneous Info: | | Virus Succeptibility: | | Virus Resistance: | | Tumor Formation: | IN C57BL/6J MOUSE | Products: | | Dependency Isoenzymes: | | Culture Medium: | Dulbecco's MEM 90%, fetal bovine serum 10%. Standard trypsinization
techniques. Cultures may be passaged by scraping the cells from the
flask, dispersing them by rapid pipetting and seeding into new flasks
with fresh medium. | Consigner: | Instituto Nacional do Cancer, Rio de Janeiro. | Reference: | Nature New Biol. 242: 148-149, 1973;
Cancer Res. 35: 218, 1975;
Cancer Res. 36: 3608, 1976;
Cancer Res. 37: 3945, 1977;
J. Nat. Cancer Inst. 60: 1217-22, 1978. | Additional Info: | The B16 melanoma cell line was extensively used for studies
of cancer metastasis. The B16-F1 line is a standard clone
usually compared with B16-F10 clone that was selected for its
high capacity to form lung metastasis. | ATCC: | CRL-6323 | Biosafety: | 01 |
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