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BCRJ: | 0043 | Cell Line: | B-13 | Tissue: | HEMATOPOIETIC | Organ: | | Cell Type: | LYMPHOBLAST | Morphology: | LYMPHOBLAST | Pathology: | | Scientific Name: | | Vulgar Name: | | Sex: | | Miscelaneous Info: | | Virus Succeptibility: | | Virus Resistance: | | Tumor Formation: | | Products: | | Dependency Isoenzymes: | Interleukin-5; IL-5 | Culture Medium: | RPMI-1640 medium 90%, fetal bovine serum 10%, and a source of IL-5.
Subculture procedure: maintain culture from 1x10E5 to 1x10E6 cells/mL.
Further information on request. | Consigner: | Dr. Paola Minoprio, Institute Pasteur, Paris; Through Dr. Radovan
Borojevic, Instituto de Quimica, Departamento de Bioquimica, UFRJ,
Rio de Janeiro. | Reference: | | Additional Info: | B13 cell line is dependent upon IL-5. It can be used to quantify the
presence of IL-5 in biologiccal fluids. This cell line can spontaneously
Loose the dependence upon IL-5, and this should be tested for each
batch of cells. The origin of this cell line should be acknowledge in all
relevant publications. May be distributed to scientific institutions; not
to be distributed for any commercial use. | ATCC: | | Biosafety: | 01 |
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