Results of Search

BCRJ:	0041
Cell Line:	ARPE-19
Tissue:	RETINA
Organ:	EYE
Cell Type:	EPITHELIAL
Morphology:	EPITHELIAL
Pathology:
Scientific Name:	HOMO SAPIENS
Vulgar Name:	HUMAN
Sex:	MALE
Miscelaneous Info:
Virus Succeptibility:
Virus Resistance:
Tumor Formation:
Products:
Dependency Isoenzymes:
Culture Medium:	A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium and 0.5 mM sodium pyruvate, 90%; fetal bovine serum, 10% Subculture procedure: standard trypsinization techniques. A split ratio of 1:3 to 1:6 is recomended. Medium renewal: 1 to 2 times per week Temperature: 37.0C
Consigner:	Kátia da Silva Calabrese Instituto Oswaldo Cruz - Fundação Oswaldo Cruz - Department of Protozoology - Laboratory Immunemodulation
Reference:	Dunn KC , et al. ARPE-19, A human retinal pigment epithelial cell line with differentiated properties. Exp. Eye Res. 62: 155-169, 1996. PubMed: 8698076 48288: Maidji E , et al. Accessory human cytomegalovirus glycoprotein US9 in the unique short component of the viral genome promotes cell-to-cell transmission of virus in polarized epithelial cells. J. Virol. 70: 8402-8410, 1996. PubMed: 8970961 48289: Holtkamp GM , et al. Polarized secretion of IL-6 and IL-8 by human retinal pigment epithelial cells. Clin. Exp. Immunol. 112: 34-43, 1998. PubMed: 9566787 48291: Finnemann SC , et al. Phagocytosis of rod outer segments by retinal pigment epithelial cells requires alpha(v)beta5 integrin for binding but not for internalization. Proc. Natl. Acad. Sci. USA 94: 12932-12937, 1997.
Additional Info:	Organ: eye - Tissue: retinal pigmented epithelium; retina - Disease: normal ARPE-19 is a spontaneously arising retinal pigment epithelia (RPE) cell line derived in 1986 by Amy Aotaki-Keen from the normal eyes of a 19-year-old male who died from head trauma in a motor vehicle accident. The cells were subjected to selective trypsinization for the first four passages to remove superficial cells before passaging the cuboidal basal layer. By passage 5, the cultures appeared to be rapidly growing RPE cells, which would form cobblestone monolayers, which pigmented after several months in culture. The line was established in a 1:1 mixture of Dulbecco's modified Eagles medium and Ham's F12 medium with HEPES buffer containing 20% fetal bovine serum, 56 mM final concentration sodium bicarbonate and 2 mM L-glutamine and incubated at 37C in 10% CO2. These cells form stable monolayers, which exhibit morphological and functional polarity. ARPE-19 expresses the RPE-specific markers CRALBP and RPE-65.
ATCC:	CRL-2302
Biosafety:	01