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| BCRJ: | 0040 | | Cell Line: | AML-193 | | Tissue: | | | Organ: | PERIPHERAL BLOOD | | Cell Type: | MONOCYTE | | Morphology: | LYMPHOBLAST | | Pathology: | | | Scientific Name: | HOMO SAPIENS | | Vulgar Name: | HUMAN | | Sex: | FEMALE | | Miscelaneous Info: | | | Virus Succeptibility: | | | Virus Resistance: | | | Tumor Formation: | | | Products: | | | Dependency Isoenzymes: | | | Culture Medium: | Complete growth medium: Iscove's modified Dulbecco's medium with 0.005 mg/ml insulin, 0.005 mg/ml transferrin and 5 ng/ml GM-CSF, 95%; fetal bovine serum, 5%.
Growth Conditions: The cells are dependent upon GM-CSF for long term growth. Conditioned medium from U-87MG may be used at a concentration of 10% to replace the GM-CSF in the medium (see J. Immunol. 139:3348- 3354, 1987).
Subculturing: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 X 10(5) cells/ml and maintain between 3 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Storage temperature: liquid nitrogen vapor phase | | Consigner: | CIALLYX
CRISTINA MASSOCO | | Reference: | 22617: Lange B, et al. Growth factor requirements of childhood acute leukemia: establishment of GM-CSF-dependent cell lines. Blood 70: 192-199, 1987. PubMed: 3496132
23467: Santoli D, et al. Synergistic and antagonistic effects of recombinant human interleukin (IL) 3, IL-1 alpha, granulocyte and macrophage colony-stimulating factors (G-CSF and M-CSF) on the growth of GM-CSF-dependent leukemic cell lines. J. Immunol. 139: 3348-3354, 1987. PubMed: 3500218 | | Additional Info: | Interleukin-3 (interleukin 3, IL-3) and granulocyte/macrophage colony stimulating factor (GM-CSF) act synergistically to stimulate growth of the cells. Granulocyte colony stimulating factor (G-CSF) also supports short term and long term growth of AML-193 cells and acts synergistically with GM-CSF in inducing proliferation of the cells.
Antigen Expression:
CD15, human; Homo sapiens, expressed (75 to 95% of the cells) (75 to 95% of the cells)
DNA Profile (STR):
Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 12
D5S818: 9,13
D7S820: 9,12
THO1: 7,9
TPOX: 8,9
vWA: 15,17
Cytogenetic Analysis:
This is a hyperdiploid human cell line. The modal chromosome number is 49, occurring in 50% of cells. The rate of polyploidy is 2.2%. There is one marker chromosome, der(17)t(17;17)(p13.1;q21.3), that occurred in every cell. These cells are trisomic for N3, N6, N8, and N13, and occasionally also for N2. N17 and X chromosome had a single copy, but occasionally the X had two copies. Thus the modal karyotype of this cell line is 49, X, +3, +6, +8, +der(17)t(17;17)(p13.1;q21.3), -17. The subline with 50, X, +2, +3, +6, +8, +der(17)t(17;17)(p13.1;q21.3), -17 and 50, XX, +3, +6, +8, +der(17)t(17;17)(p13.1;q21.3, -17 also occurred, but at a very low frequency. | | ATCC: | CRL-9589 | | Biosafety: | 1 |
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