| BCRJ: | 0039 |
| Cell Line: | AMJ2-C11 |
| Tissue: | |
| Organ: | LUNG |
| Cell Type: | MACROPHAGE |
| Morphology: | MACROPHAGE-LIKE |
| Pathology: | |
| Scientific Name: | MUS MUSCULUS |
| Vulgar Name: | MOUSE |
| Sex: | FEMALE |
| Miscelaneous Info: | Antigen Expression: MAC-1 (CD11b) +; MAC-2 +; Fc receptor (FcR) +; Ly-5 +; Thy-1 -; Lyt-1 - |
| Virus Succeptibility: | |
| Virus Resistance: | |
| Tumor Formation: | |
| Products: | interleukin-6 (interleukin 6, IL-6) |
| Dependency Isoenzymes: | |
| Culture Medium: | Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 5 mM HEPES, 95%; fetal bovine serum, 5%.
Scrape off the attached cells and transfer along with the floating cells into new flasks. |
| Consigner: | Antonio Martins Monteiro - Rio de Janeiro Cell Bank - Rio de Janeiro Federal University - University Hospital Clementino Fraga Filho |
| Reference: | 48933: Palleroni AV , et al. Tumoricidal alveolar macrophage and tumor infiltrating macrophage cell lines. Int. J. Cancer 49: 296-302, 1991. PubMed: 1879973
48934: Palleroni AV , et al. Nitric oxide synthase induction in lines of macrophages from different anatomical sites. Cell. Mol. Biol. 44: 527-535, 1998. PubMed: 9620450 |
| Additional Info: | Cell type: macrophage (alveolar); infected with J2 virus.
AMJ2-C8 (ATCC CRL-2455) and AMJ2-C11 (ATCC CRL-2456) are cloned, continuous, alveolar macrophage (AM) cell lines generated from C57BL6J mice by in vitro infection with the J2 retrovirus carrying the v-raf and v-myc oncogenes. [48933]
Flow cytometry detected the product of the raf gene in the cytoplasm of these cell lines.
Studies on the tumoricidal properties of these cell lines demonstrated differences in their response to a panel of known macrophage activators.
AMJ2-C8 was activated following exposure to recombinant murine interferon gamma (rMuIFN-gamma) but not lipopolysaccharide (LPS) or muramyl dipeptide (MDP).
AMJ2-C11 most closely resembled the response pattern of the parental AM, since it could be activated by either the combination of rMuIFN-gamma plus LPS or rMuIFN-gamma plus MDP.
The cells retain many characteristics of alveolar macrophages. They are phagocytic, non-specific esterase positive and they express macrophage Mac-1 antigens and Fc receptors.
Constitutive expression of MHC-class-II antigens was low on AMJ2-C11 but was increased following exposure to rMuIFN-gamma.
The cell line did not secrete substantial amounts of IL-1 or TNF but did secrete large amounts of IL-6.
The cells produce nitric oxide (NO) when stimulated with a mixture of rMuIFN-gamma and LPS |
| ATCC: | CRL-2456 |
| Biosafety: | 01 |
