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Results of Search
| BCRJ: | 0038 | | Cell Line: | AFT024 | | Tissue: | | | Organ: | LIVER | | Cell Type: | FIBROBLAST | | Morphology: | FIBROBLAST | | Pathology: | | | Scientific Name: | MUS MUSCULUS | | Vulgar Name: | MOUSE | | Sex: | | | Miscelaneous Info: | | | Virus Succeptibility: | | | Virus Resistance: | | | Tumor Formation: | | | Products: | | | Dependency Isoenzymes: | | | Culture Medium: | Complete growth medium: The base medium for this cell line is Dulbecco's Modified Eagle's Medium. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 33.0°C
Max Temperature: 33.0°C
Min Temperature: 31.0°C
Subcultivation Ratio: 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 40 to 48 hrs | | Consigner: | MARIA ISABEL ROSSI
KINCADE | | Reference: | 29080: Moore KA, et al. Hematopoietic activity of a stromal cell transmembrane protein containing epidermal growth factor-like repeat motifs. Proc. Natl. Acad. Sci. USA 94: 4011-4016, 1997. PubMed: 9108096
39171: Miller JS, et al. Single adult human CD34(+)/Lin-/CD38(-) progenitors give rise to natural killer cells, B-lineage cells, dendritic cells, and myeloid cells. Blood 93: 96-106, 1999. PubMed: 9864151
70208: Moore KA, et al. In vitro maintenance of highly purified, transplantable hematopoietic stem cells. Blood 89: 4337-4347, 1997. PubMed: 9192756
70209: Hachney JA, et al. A molecular profile of a hematopoietic stem cell niche. Proc. Natl. Acad. Sci. USA 99: 13061-13066, 2002. PubMed: 12226475
70210: Punzel M, et al. The myeloid-lymphoid initiating cell (ML-IC) assay assesses the fate of multipotent human progenitors in vitro. Blood 93: 3750-3756, 1999. PubMed: 10339481
70213: Thiemann FT, et al. The murine stromal cell line AFT024 acts specifically on human CD34+CD38- progenitors to maintain primitive function and immunophenotype in vitro. Exp. Hematol. 26: 612-619, 1998. PubMed: 9657136
70214: Lewis ID, et al. Umbilical cord blood cells capable of engrafting in primary, secondary, and tertiary xenogeneic hosts are preserved after ex vivo culture in a noncontact system. Blood 97: 3441-3449, 2001. PubMed: 11369635 | | Additional Info: | This cell line, immortalized with temperature sensitive SV-40 T antigen, was derived from murine fetal liver stromal cells. They represent cells obtained from the microenvironment of the liver that support purified mouse and human CD34+CD38- hematopoietic stem/progenitor cells. Delta-like (Dlk1) /preadipocyte factor-1 (pref-1) is preferentially expressed in AFT024 cells and functions as a positive stem cell regulator. Due to the presence of the temperature sensitive SV40 T antigen, the AFT024 cells must be grown at 33C. At 33C, T antigen binds anti-oncogenes and allows cell division. At higher temperatures, such as 37C, T antigen is inactivated and the cells can no longer divide. SCRC-1007 can serve as a support layer for stem cells at 37C; however, to insure that all AFT024 cells are mitotically inactive, irradiation or Mitomycin C treatment is recommended. | | ATCC: | SCRC-1007 | | Biosafety: | 2 |
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