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BCRJ: | 0032 | Cell Line: | A-431 | Tissue: | EPITHELIUM | Organ: | SKIN | Cell Type: | B LYMPHOCYTE | Morphology: | EPITHELIAL | Pathology: | CARCINOMA EPIDERMOID | Scientific Name: | HOMO SAPIENS | Vulgar Name: | _HUMAN | Sex: | FEMALE | Miscelaneous Info: | | Virus Succeptibility: | | Virus Resistance: | | Tumor Formation: | YES | Products: | | Dependency Isoenzymes: | | Culture Medium: | The base medium for this cell line is Dulbecco's Modified Eagle's Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days | Consigner: | RENATA F. V. LOPES
FCFRB | Reference: | 23093: Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216
23218: Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758
32507: Kovelman R, et al. Enhanced transcriptional activation by E2 proteins from the oncogenic human papillomaviruses. J. Virol. 70: 7549-7560, 1996. PubMed: 8892874
32568: Lee JH, et al. The proximal promoter of the human transglutaminase 3 gene. J. Biol. Chem. 271: 4561-4568, 1996. PubMed: 8626812
32582: Chang K, Pastan I. Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers. Proc. Natl. Acad. Sci. USA 93: 136-140, 1996. PubMed: 8552591
32912: Wizemann TM, et al. Peptide methionine sulfoxide reductase contributes to the maintenance of adhesins in three major pathogens. Proc. Natl. Acad. Sci. USA 93: 7985-7990, 1996. PubMed: 875558 | Additional Info: | The epidermoid carcinoma cell line A-431, derived from an 85-year-old female, is one of a series of cell lines established from solid tumors by D.J. Giard, et al. [23218]
DNA Profile (STR):
Amelogenin: X
CSF1PO: 11,12
D13S317: 9,13
D16S539: 12,14
D5S818: 12,13
D7S820: 10
THO1: 9
TPOX: 11
vWA: 15,17
Cytogenetic Analysis: This is a hypertriploid human cell line. The modal chromosome number was 74 occurring in 36% of cells. The rate of cells with higher ploidies was 1.0%.
Isoenzymes:
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 0
PGM1, 1
PGM3, 1 | ATCC: | CRL-1555 | Biosafety: | 1 |
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