Universidade Federal do Rio de JaneiroRJCB Collection
Home Page

Results of Search

BCRJ: 0032
Cell Line: A-431
Tissue: EPITHELIUM
Organ: SKIN
Cell Type: B LYMPHOCYTE
Morphology: EPITHELIAL
Pathology: CARCINOMA EPIDERMOID
Scientific Name: HOMO SAPIENS
Vulgar Name: _HUMAN
Sex: FEMALE
Miscelaneous Info:
Virus Succeptibility:
Virus Resistance:
Tumor Formation: YES
Products:
Dependency Isoenzymes:
Culture Medium: The base medium for this cell line is Dulbecco's Modified Eagle's Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. A subcultivation ratio of 1:3 to 1:8 is recommended Medium Renewal: Every 2 to 3 days
Consigner: RENATA F. V. LOPES FCFRB
Reference: 23093: Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216 23218: Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758 32507: Kovelman R, et al. Enhanced transcriptional activation by E2 proteins from the oncogenic human papillomaviruses. J. Virol. 70: 7549-7560, 1996. PubMed: 8892874 32568: Lee JH, et al. The proximal promoter of the human transglutaminase 3 gene. J. Biol. Chem. 271: 4561-4568, 1996. PubMed: 8626812 32582: Chang K, Pastan I. Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers. Proc. Natl. Acad. Sci. USA 93: 136-140, 1996. PubMed: 8552591 32912: Wizemann TM, et al. Peptide methionine sulfoxide reductase contributes to the maintenance of adhesins in three major pathogens. Proc. Natl. Acad. Sci. USA 93: 7985-7990, 1996. PubMed: 875558
Additional Info: The epidermoid carcinoma cell line A-431, derived from an 85-year-old female, is one of a series of cell lines established from solid tumors by D.J. Giard, et al. [23218] DNA Profile (STR): Amelogenin: X CSF1PO: 11,12 D13S317: 9,13 D16S539: 12,14 D5S818: 12,13 D7S820: 10 THO1: 9 TPOX: 11 vWA: 15,17 Cytogenetic Analysis: This is a hypertriploid human cell line. The modal chromosome number was 74 occurring in 36% of cells. The rate of cells with higher ploidies was 1.0%. Isoenzymes: AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 0 PGM1, 1 PGM3, 1
ATCC: CRL-1555
Biosafety: 1


© 2000 Rio de Janeiro - Cell Bank / UFRJ
Conectada à INTERNET através da RedeRio de computadores
Webmaster RJCB