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| BCRJ: | 0030 | | Cell Line: | A20 | | Tissue: | HEMATOPOIETIC | | Organ: | | | Cell Type: | MYELOID | | Morphology: | MYELOID | | Pathology: | MYELOMA | | Scientific Name: | MUS MUSCULUS | | Vulgar Name: | MOUSE; BALB/C ANN
SEX | | Sex: | | | Miscelaneous Info: | | | Virus Succeptibility: | | | Virus Resistance: | | | Tumor Formation: | IN MICE | | Products: | | | Dependency Isoenzymes: | | | Culture Medium: | RPMI-1640medium 90% with 0,05mM 2-mercaptoethanol, fetal bovine serum 10%.
Subculture procedure: maintain cultures from 1x10E5 to 1x10E6 cells/mL. | | Consigner: | Dr. Elizabeth Obino Lima, Fundaçao Instituto Oswaldo Cruz, Rio de Janeiro. | | Reference: | Immunopharmacology 28(3): 183-92, 1994;
J. Immunol. 154(4): 1699-706, 1995 | | Additional Info: | A20 is a Balb/c B cell lymphoma line derived from a spontaneous reticulum cell
neoplasm found in an old ( older than 15 months ) Balb/c AnN mouse. These
cells are IgG+, Ig+ ( with a polivalent anti-Ig ), Ia+, Fc+, IgM -, IgA-, and
complement receptor negative. The A20 cell line is diploid, tumorogenic in mice
and have a generation time of 18 hours. Cells originally expressed very little
surface IgG when they were grown in ClickÆs medium; however, when cells are
grown in RPMI-1640 they express a high level of surface IG. T cell factors and
mitogens can induce these cells to secrete IgG extracellularly. This Ia antigen
positive B cell tumor line os capable of presenting both alloantigens and protein
antigens to alloreactive and antigen reactive T-lymphocytes in a major
histocompatibility complex restricted fashion. The A20 cells, by comparison with
conventional antigen presenting cells, should prove useful in studying the
biochemical events that occur during antigen processing and the requirements
for T cell triggering by processed antigen in association with Ia molecules. May be
distributed to scientific institutions; not to distributed for any commercial use. | | ATCC: | TIB208 | | Biosafety: | 01 |
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