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| BCRJ: | 0025 | | Cell Line: | 53-7.313 | | Tissue: | HEMATOPOIETIC | | Organ: | | | Cell Type: | LYMPHOBLAST | | Morphology: | LYMPHOBLAST | | Pathology: | HYBRIDOMA | | Scientific Name: | RATTUS NORVEGICUS | | Vulgar Name: | RAT; LOU/WS1/M | | Sex: | | | Miscelaneous Info: | | | Virus Succeptibility: | | | Virus Resistance: | | | Tumor Formation: | | | Products: | Immunoglobulin; monoclonal antibody; Ly-1murine: ab against; IgG | | Dependency Isoenzymes: | | | Culture Medium: | Dulbecco's MEM 4.5g/L glucose 90%, fetal bovine serum 10%.
Subculture procedures: maintain cultures from 1x10E5 to 1x10E6 cells/mL. | | Consigner: | Dr. Alberto Nobrega, Instituto de Microbiologia, CCS, UFRJ,
Rio de Janeiro. | | Reference: | Immunol. Rev. 47: 63-90, 1979. | | Additional Info: | This hybridoma secretes a rat IgG2a antibody reactive with the
lymphocyte differentiation antigen Lyt-1 ( all alleles ). Fluorescent
staining and fluorescent-activated cell sorter analysis show that this
antibody is reactive with all thymocytes ( > 98% ) and with T cells in the
spleen ( 35% ). Less than 1% in bone marrow cells, 63% of peripheral
blood lymphocytes and 89% of lymph node cells were reactive. These
antibodies are citotoxic after conjugation with arsanilic acid and can be
used with a rabbit anti-arsanilic acid antibody for sandwich killing. This
hybridoma was generated by the fusion of the mouse myeloma line
NS-1 with spleen cells of LOU/Ws1/M rats imunized with mouse thymus
or spleen. | | ATCC: | TIB 104 | | Biosafety: | 01 |
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