| BCRJ: | 0023 |
| Cell Line: | 53-6.72 |
| Tissue: | HEMATOPOIETIC |
| Organ: | |
| Cell Type: | LYMPHOBLAST |
| Morphology: | LYMPHOBLAST |
| Pathology: | HYBRIDOMA |
| Scientific Name: | RATTUS NORVEGICUS |
| Vulgar Name: | RAT; LOU/WS1/M |
| Sex: | |
| Miscelaneous Info: | |
| Virus Succeptibility: | |
| Virus Resistance: | |
| Tumor Formation: | |
| Products: | Immunoglobulin; monoclonal antibody; CD8 murine: ab aginst; IgM |
| Dependency Isoenzymes: | |
| Culture Medium: | RPMI-1640 medium 90%, fetal bovine serum 10%.
Subculture procedure: maintain cultures from 1x10E5 to 1x10E6 cells/mL. |
| Consigner: | Banco de Células do Rio de Janeiro |
| Reference: | Immunol. Rev. 47:63-90, 1979. |
| Additional Info: | This hybridoma secretes a rat anti-mouse IgG2a antibody which is
reactive with the lymphocyte diferentiantion antigen Lyt-2 ( all alleles ).
Fluorescence staining and fluorescence-activated cell sorter analysis
show that this antibody is reactive with 90% of thymocytes and with T
cells in the spleen ( 11% ). Less than 1% of bone marrow cells, 12% of
peripheral blood lymphocytes, and 25% of lymp nodes cells were
reactive. These antibodies are cytotoxic after conjugation with arsanilic
acid and use of rabbit anti-arsanilic acid antibody for sandwich killing.
Spleen cells from LOU/Ws1/M rat imunized with mouse thymus or
spleen were fused with the mouse myeloma line NS-1 to originate this
hybridoma. |
| ATCC: | TIB 105 |
| Biosafety: | 01 |
