| BCRJ: | 0020 |
| Cell Line: | 3T6 - SWISS ALBINO |
| Tissue: | EMBRYO |
| Organ: | |
| Cell Type: | FIBROBLAST |
| Morphology: | FIBROBLAST |
| Pathology: | |
| Scientific Name: | MUS MUSCULUS |
| Vulgar Name: | MOUSE; SWISS ALBINO |
| Sex: | |
| Miscelaneous Info: | |
| Virus Succeptibility: | HERPES SIMPLEX; VACCINIA; PSEUDORABIES VESICULAR S |
| Virus Resistance: | POLIOVIRUS 2 |
| Tumor Formation: | |
| Products: | Collagen; Hyaluronic acid |
| Dependency Isoenzymes: | |
| Culture Medium: | Dulbecco's MEM 90%, fetal bovine serum 10%.
Subculture procedure: standard trypsinization techniques.
A split ratio 1:4 is recommended. |
| Consigner: | Dr. Hugo Armelin, Departamento Bioquimica, ICB, USP, Sao Paulo. |
| Reference: | J. Cell Biol. 17: 299-313, 1963. |
| Additional Info: | The 3T6 cell line is a collagen and hyaluronic acid secreting
line established by G. Todaro and H. Green in 1963 from
disaggregated Swiss mouse embryos. These cells are similar to 3T3
cell line. However, they secrete collagen and other molecules of
the extracellular matrix, and are in general considered more
differentiated than 3T3 cell line. |
| ATCC: | CCL 96 |
| Biosafety: | 01 |
