| BCRJ: | 0016 |
| Cell Line: | 3H5-1 |
| Tissue: | |
| Organ: | |
| Cell Type: | LYMPHOBLAST |
| Morphology: | LYMPHOBLAST |
| Pathology: | |
| Scientific Name: | MUS MUSCULUS |
| Vulgar Name: | MOUSE |
| Sex: | |
| Miscelaneous Info: | Source: Cell type: hybridoma: B lymphocyte |
| Virus Succeptibility: | |
| Virus Resistance: | |
| Tumor Formation: | |
| Products: | Immunoglobulin; monoclonal antibody; against a type specific determinant on Dengue virus-2) |
| Dependency Isoenzymes: | |
| Culture Medium: | Modified Dulbecco's medium, 90%; fetal bovine serum, 10%.
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Medium renewal: Every 2 to 3 days |
| Consigner: | Ada M.B.Alves |
| Reference: | 922: Henchal EA , et al. Dengue virus-specific and Flavivirus group determinants identified with monoclonal antibodies by indirect immunofluorescence. Am. J. Trop. Med. Hyg. 31: 830-836, 1982. PubMed: 6285749 |
| Additional Info: | Animals were immunized with dengue virus type 2 antigens from infected mouse brains (the prototype strain New Guinea C was used).
The antibody is type specific.
Spleen cells were fused with P3X63Ag8 myeloma cells.
Antibodies should be prepared as ascites in pristane primed BALB/c mice.
Tested and found negative for ectromelia virus (mousepox). |
| ATCC: | HB-46 |
| Biosafety: | 01 |
