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| BCRJ: | 0015 | | Cell Line: | 3G5 | | Tissue: | | | Organ: | | | Cell Type: | B LYMPHOCYTE | | Morphology: | LYMPHOBLAST | | Pathology: | | | Scientific Name: | MUS MUSCULUS | | Vulgar Name: | MOUSE | | Sex: | | | Miscelaneous Info: | | | Virus Succeptibility: | | | Virus Resistance: | | | Tumor Formation: | | | Products: | immunoglobulin; monoclonal antibody; against a ganglioside associated with endocrine cells (pancreatic islet, adrenal medulla, pituitary, thyroid), human T lymphocytes and neuronal cells | | Dependency Isoenzymes: | | | Culture Medium: | Complete growth medium: The base medium for this cell line is Dulbecco's Modified Eagle's Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Medium Renewal: Every 2 to 3 days
Storage temperature: liquid nitrogen vapor temperature | | Consigner: | Antonio Martins Monteiro - Banco de Células do Rio de Janeiro | | Reference: | 1623: Rabinowe SL, et al. Aging in man: Linear increase of a novel T cell subset defined by antiganglioside monoclonal antibody 3G5. J. Exp. Med. 165: 1436-1441, 1987. PubMed: 3494809
1624: Powers AC, et al. Characterization of monoclonal antibody 3G5 and utilization of this antibody to immobilize pancreatic islet cell gangliosides in a solid phase radioassay. Endocrinology 114: 1338-1343, 1984. PubMed: 6368201
23439: Nayak RC, et al. A monoclonal antibody (3G5)-defined ganglioside antigen is expressed on the cell surface of microvascular pericytes. J. Exp. Med. 167: 1003-1015, 1988. PubMed: 3351433
23512: Bartholomeusz RK, et al. Pancreatic islet A2B5- and 3G5-reactive gangliosides are markers of differentiation in rat insulinoma cells. Endocrinology 124: 2680-2685, 1989. PubMed: 2541995 | | Additional Info: | Animals were immunized with fetal rat brain.
Spleen cells were fused with myeloma cells.
The antibody reacts with an acetylated ganglioside common to pancreatic islet cells, neurons, adrenal medulla, thyroid follicle renal glomerulus, human T lymphocytes and a majority of cells in the pituitary.
The antibody reacts with an O-acetylated disialoganglioside present on a wide variety of endocrine and neuronal tissues. In addition, the antibody recognizes an antigen on human T lymphocytes that defines a subset of T cells that increases with age.
Increasing expression of the 3G5 reactive ganglioside correlates with increasing degree of differentiation of rat insulinoma cells (RIN5F).
The 3G5 antigen is expressed on microvascular pericyte plasma membranes, and is useful as a marker of pericytes in vitro.
Tested and found negative for ectromelia virus (mousepox). | | ATCC: | CRL-1814 | | Biosafety: | 1 |
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