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Results of Search
| BCRJ: | 0008 | | Cell Line: | 25.9.17S | | Tissue: | HEMATOPOIETIC | | Organ: | | | Cell Type: | LYMPHOBLAST | | Morphology: | LYMPHOBLAST | | Pathology: | HYBRIDOMA | | Scientific Name: | MUS MUSCULUS | | Vulgar Name: | MOUSE | | Sex: | | | Miscelaneous Info: | | | Virus Succeptibility: | | | Virus Resistance: | | | Tumor Formation: | | | Products: | Immunoglobulin; monoclonal antibody; MHC antigens: ab against; I-Ab: ab
against; IgG 2a | | Dependency Isoenzymes: | | | Culture Medium: | RPMI-1640 medium 90%, fetal bovine serum 10%.
Subculture procedure: maintain cultures from 1x10E5 to 1x10E6 cells/mL | | Consigner: | Dr. Alberto Nobrega, Instituto de Microbiologia, CCS, UFRJ,
Rio de Janeiro. | | Reference: | J.Immunol. 126: 317-321, 1981. | | Additional Info: | This hybridoma secretes a monoclonal antibody ( IgG2a kappa )
against MHC antigens, that reacts with I-Ab and I-Ad antigens.
Cross reactions with H-2p and H-2q was also found. The specificity
patterns does not correspond to any previously known I-a
specificities. This line was derived by fusing SP2/0-Ag14 myeloma
cells with lymphoid cells from C3H mouse imunized with spleen cells
from C3H.SW. | | ATCC: | | | Biosafety: | 01 |
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