| BCRJ: | 0005 |
| Cell Line: | 1F5 |
| Tissue: | |
| Organ: | |
| Cell Type: | LYMPHOBLASTOID |
| Morphology: | LYMPHOBLAST |
| Pathology: | HYBRIDOMA |
| Scientific Name: | MUS MUSCULUS |
| Vulgar Name: | MOUSE |
| Sex: | |
| Miscelaneous Info: | The antibody is specific for CD20 (expressed on normal and neoplastic human B cells) and can induce proliferation of resting B cells. |
| Virus Succeptibility: | |
| Virus Resistance: | |
| Tumor Formation: | |
| Products: | Immunoglobulin; monoclonal antibody; against human CD20 B cell antigen (Bp35) |
| Dependency Isoenzymes: | |
| Culture Medium: | RPMI 1640 medium 85%; fetal bovine serum, 15%.
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Medium renewal: Every 2 to 3 days |
| Consigner: | Márcio Poças Universidade de Brasília |
| Reference: | Senter PD , et al. Antibody-enzyme conjugates in combination with prodrugs for the delivery of cytotoxic agents to tumor cells. US Patent 4,975,278 dated Dec 4 1990
Clark EA , et al. Role of the Bp35 cell surface polypeptide in human B-cell activation. Proc. Natl. Acad. Sci. USA 82: 1766-1770, 1985. PubMed: 3872456 |
| Additional Info: | |
| ATCC: | HB-9645 |
| Biosafety: | 01 |
