| BCRJ: | 0003 |
| Cell Line: | 15.5.5S |
| Tissue: | HEMATOPOIETIC |
| Organ: | |
| Cell Type: | LYMPHOBLAST |
| Morphology: | LYMPHOBLAST |
| Pathology: | HYBRIDOMA |
| Scientific Name: | MUS MUSCULUS |
| Vulgar Name: | MOUSE |
| Sex: | |
| Miscelaneous Info: | |
| Virus Succeptibility: | "Immunoglobulin; monoclonal antibody; MHC antigens: ab against; H-2 Kd,Dd: |
| Virus Resistance: | |
| Tumor Formation: | |
| Products: | Immunoglobulin; monoclonal antibody; MHC antigens: ab against; H-2 Kd,Dd:
ab against |
| Dependency Isoenzymes: | |
| Culture Medium: | RPMI-1640 medium 90%, fetal bovine serum 10%.
Subculture procedure: maintain cultures from 1x105 to 1x106 cells/mL. |
| Consigner: | Dr. Alberto Nobrega, Instituto de Microbiologia, CCS, UFRJ,
Rio de Janeiro. |
| Reference: | J.Immunol. 124:533-540, 1980. |
| Additional Info: | This hybridoma produces a cytotoxic monoclonal antibody
( IgG2a kappa ) that reacts with Dk antigens. It also reacts with Kd
and Kf. The reactivity is unlike any known H-2 published specificity.
The line was developed by fusion of Sp2/0-Ag14 cells with spleen
cells of C3H.SW mice imunized with C3H splenocytes. |
| ATCC: | HB-24 |
| Biosafety: | 01 |
